| 货号 | 8970S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from various cell lines using PAX5 (D19F8) XP® Rabbit mAb. 用PAX5 (D19F8) XP® 兔单抗对多种细胞提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of Ramos (left) and Jurkat (right) cells using PAX5 (D19F8) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用PAX5 (D19F8) XP®兔单抗（绿色）和 β-Actin (8H10D10) 鼠单抗#3700（红色）对Ramos（左）和Jurkat（右）细胞进行激光共聚焦荧光分析。蓝色伪彩=DRAQ5®4084（DNA荧光染料） | |
Flow cytometric analysis of Jurkat (red) and Ramos (blue) cells using PAX5 (D19F8) XP® Rabbit mAb. 用 PAX5 (D19F8) XP®兔单抗对Jurkat（红色）和Ramos（蓝色）细胞进行流式细胞分析。 |
