| 货号 | 3550S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | PIAS1 (D33A7) XP® Rabbit mAb detects endogenous levels of total PIAS1 protein. |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | The PIAS (protein inhibitor of activated Stat) proteins, which include PIAS1, PIAS3, PIASx, and PIASy, were originally characterized based on their interaction with the Stat family of transcription factors (1,2). PIAS1, PIAS3, and PIASx interact with and repress Stat1, Stat3, and Stat4, respectively (1-3). Deletion of PIAS1 leads to inhibition of interferon-inducible genes and increased protection against infection (4). The PIAS family contains a conserved RING domain that has been linked to a function as a SUMO (small ubiquitin-related modifier) ligase, coupling the SUMO conjugating enzyme Ubc9 with its substrate proteins (5,6). Numerous studies have now shown that PIAS family members can regulate the activity of transcription factors through distinct mechanisms, including NF-κB (7,8), c-Jun, p53 (5,9), Oct-4 (11), and Smads (11,12). The activity of PIAS1 is regulated by both phosphorylation and arginine methylation. Inflammatory stimuli can induce IKK-mediated phosphorylation of PIAS1 at Ser90, which is required for its activity (13). In addition PRMT1 induces arginine methylation of PIAS1 at Arg303 following interferon treatment and is associated with its repressive activity on Stat1 (14). |
| 存放说明 | -20C |
| 计算分子量 | 76 |
Flow cytometric analysis of HeLa cells using PIAS1 (D33A7) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).流式细胞仪分析HeLa细胞，所用抗体为PIAS1 (D33A7) XP® Rabbit mAb (蓝色) 与非特异性阴性对照抗体对比(红色)。 | |
Confocal imunofluorescent analysis of HeLa cells using PIAS1 (D33A7) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).共聚焦免疫荧光分析HeLa细胞，所用抗体为PIAS1 (D33A7) XP® Rabbit mAb (绿色)。肌动蛋白丝用DY-554 phalloidin标记 (红色)。 | |
Western blot analysis of extracts from various cell lines using PIAS1 (D33A7) XP® Rabbit mAb.Western免疫印迹。用PIAS1 (D33A7) XP® Rabbit mAb研究各种细胞系的细胞提取液。 |
