RIP (D94C12) XP® Rabbit mAb【科瑞奥博】

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RIP (D94C12) XP® Rabbit mAb

货号： 3493S 基本售价： 3860.0 元规格： 100ul

产品信息

概述
货号	3493S
反应种属	Human/Mouse/Rat/Hamster/Monkey
来源宿主	Rabbit
应用	W/IP/IF-IC/F
目标/特异性	RIP (D94C12) XP® Rabbit mAb detects endogenous levels of total RIP (RIP1) protein. It has not been shown to cross-react with other RIP family members.
使用方法	WB(1:1000) IP (1:100) F (1:200) IF-IC (1:100)

性能
供应商	CST
背景	The RIP (receptor-interacting protein) family of serine-threonine kinases (RIP, RIP2, RIP3 and RIP4) are important regulators of cellular stress that can trigger pro-survival and inflammatory responses through the activation of NF-κB as well as pro-apoptotic pathways (1). In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and for the recruitment to TNFR1 through interaction with TRADD (2,3). RIP also interacts with TNF-receptor-associated factors (TRAFs) and can recruit IKKs to the TNFR1 signaling complex via interaction with NEMO leading to IκB phosphorylation and degradation (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (2,3). Caspase-8 dependent cleavage of the death domain on RIP can trigger the apoptotic activity of RIP (8). RIP-deficient cells show a failure in TNF-mediated NF-κB activation making the cells more sensitive to apoptosis (4,5).
存放说明	-20C
计算分子量	78

参考图片

Western blot analysis of extracts from various cell lines using RIP (D94C12) XP® Rabbit mAb.Western免疫印迹。用RIP (D94C12) XP® Rabbit mAb研究各种细胞系的细胞提取液。

Western blot analysis of extracts from HeLa cells, untransfected or transfected with human RIP construct, using RIP (D94C12) XP® Rabbit mAb.Western免疫印迹。用RIP (D94C12) XP® Rabbit mAb研究未转染的和转染了含有人RIP基因的质粒的HeLa细胞的细胞提取液。

Confocal immunofluorescent analysis of OVCAR8 cells using RIP (D94C12) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析OVCAR8细胞，所用抗体为RIP (D94C12) XP® Rabbit mAb (绿色)。Blue pseudocolor = DRAQ5® #4084 (DNA 荧光染料)。

Flow cytometric analysis of MCF7 cells using RIP (D94C12) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).流式细胞仪分析MCF7细胞，所用抗体为RIP (D94C12) XP® Rabbit mAb (蓝色)与Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (红色)做对比。