| 货号 | 8509S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | TIAR (D32D3) XP® Rabbit mAb recognizes endogenous levels of total TIAR protein. This antibody also weakly recognizes recombinant TIA1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:400) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | TIAR is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins (1,2). It functions as a translational repressor under conditions of cellular damage (3,4). In response to cellular stress, TIAR associates with eIF1, eIF3, and the 40S ribosomal subunit and forms noncanonical preinitiation complexes that are translationally inactive (3,4). TIAR then aggregates with its family member TIA1 and facilitates the accumulation of the translationally inactive preinitiation complexes into discrete cytoplasmic foci called stress granules. The two major isoforms of TIAR are the products of alternative mRNA splicing (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using TIAR (D32D3) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用TIAR (D32D3) XP® Rabbit mAb兔单抗（绿色）标记，共聚焦免疫荧光观察TIAR蛋白在HeLa细胞中定位，细胞分为对照组 (左图)和UV照射组(右图)。DY-554 phalloidin标记微丝蛋白（红色）。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料). | |
Western blot analysis of extracts from various cell lines using TIAR (D32D3) XP® Rabbit mAb. 使用TIAR (D32D3) XP® Rabbit mAb兔单抗，免疫Western blot分析不同细胞系中TIAR (D32D3)蛋白水平。 | |
Flow cytometric analysis of HeLa cells using TIAR (D32D3) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 与Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (红色)比较，使用TIAR (D32D3) XP® Rabbit mAb兔单抗 (蓝色)标记，流式细胞仪分析HeLa细胞。 |
