| 货号 | 11948S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) recognizes endogenous levels of total TNF-α protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3). |
| 存放说明 | -20C |
| 计算分子量 | 17,25,28 |
Confocal immunofluorescent analysis of Raw 264.7 cells, treated with LPS (100 ng/mL, 6 hr; left) or untreated (right), using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦分析Raw264.7细胞，LPS (100 ng/mL, 6 小时; 左图)或未处理(右图)，使用抗体是TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (绿色)。肌动蛋白微丝是通过DY-554 phalloidin标记的(红色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 | |
Immunoprecipitation of TNF-α from Raw 264.7 cell extracts, treated with LPS (100 ng/mL, 6 hr) and Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific). Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) #3678 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as sencondary antibodies.对Raw264.7细胞提取物中的TNF-α进行免疫沉淀，LPS (100 ng/mL, 6小时) 和Brefeldin A #9972 (300 ng/mL, 刺激后3小时)处理，使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或 TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (泳道3)。泳道1为10%的加入量。Western blot分析使用的抗体是TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific)。Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) #3678 和Anti-mouse IgG, HRP-linked Antibody #7076都被用来作为二抗。 | |
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated (+) with LPS (100 ng/mL, 6 hr) and Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation), using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析Raw264.7细胞的细胞提取物，未处理(-)或LPS (100 ng/mL, 6 小时) 和Brefeldin A #9972 (300 ng/mL,最后3小时刺激)，使用抗体是TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (上图)或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。 | |
Western blot analysis of 1 ng recombinant mouse TNF-α #5178 and 1 ng recombinant rat TNF-α using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific).Western blot分析 1 ng recombinant mouse TNF-α #5178 和 1 ng recombinant rat TNF-α，使用抗体是TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific)。 | |
Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of treatment) (green), using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.流式细胞术对Raw264.7进行分析，未处理(蓝色)或LPS (100 ng/ml, 6小时) 和Brefeldin A #9972 (300 ng/ml, 最后3小时处理) (绿色)，使用抗体是TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific)。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414用来作为二抗。 |
