| 货号 | 97450S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | W F |
| 目标/特异性 | IFN-β1 (D2J1D) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total IFN-β1 protein. |
| 使用方法 | WB(1:1000) F (1:3200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The type I interferon (IFN) family includes IFN-β1 and IFN-α1 through IFN-α13 in humans and IFN-α1 through IFN-α14 in mice. Type I IFN is produced following detection of pathogen-associated molecular patterns (PAMPs) and is important for induction of antiviral genes, activation of dendritic cells, and initiation of adaptive immunity (1, 2). Type I IFNs signal through the IFN alpha receptor (IFNAR), which is a heterodimer composed of IFNAR1 and IFNAR2. Activation of IFNAR leads to formation of the nuclear complex IFN-stimulated gene factor 3 (ISGF3), which consists of STAT1, STAT2, and IRF-9 (3, 4). ISGF3 binds to IFN-stimulated response elements (ISREs) to initiate transcription of interferon-stimulated genes (3). |
| 存放说明 | -20C |
| 计算分子量 | 24, 26 |
| 参考文献 | 1 . Paludan, S.R. and Bowie, A.G. (2013) Immunity 38, 870-80. 2 . Ivashkiv, L.B. and Donlin, L.T. (2014) Nat Rev Immunol 14, 36-49. 3 . Levy, D.E. et al. (1989) Genes Dev 3, 1362-71. 4 . Qureshi, S.A. et al. (1995) Proc Natl Acad Sci U S A 92, 3829-33. |
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with DMXAA (50 μg/ml, 7 hr; +), using IFN-β1 (D2J1D) Rabbit mAb (Mouse Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Brefeldin A #9972 (300 ng/ml) was added to untreated and treated cells 1 hr after the start of DMXAA treatment. | |
Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with DMXAA (50 μg/ml, 7 hr) (green), using IFN-β1 (D2J1D) Rabbit mAb (Mouse Specific). Brefeldin A #9972 (300 ng/ml) was added to untreated and treated cells 1 hr after the start of DMXAA treatment. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4112 was used as a secondary antibody. |
