| 货号 | 97249S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IHC-P IF-IC F |
| 目标/特异性 | ERG (A7L1G) Rabbit mAb recognizes endogenous levels of total ERG protein. Based on sequence identity, this antibody should detect isoforms ERG1, ERG2 and ERG3. This antibody does not cross-react with Fli1. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:200) F (1:50) IF-IC (1:500) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | ETS-related gene (ERG) is a member of the E-26 transformation-specific (ETS) family of sequence-specific DNA-binding transcription factors (1). ERG plays important and highly conserved roles in vertebrate development. Early in embryonic development, ERG is highly expressed in the embryonic mesoderm and endothelium, where it plays a critical role in the formation of the vascular system, urogenital tract and bone development (2,3). Later in embryonic development, ERG functions to regulate the pluripotency of hematopoietic stem cells, endothelial cell homeostasis and angiogenesis (2,4-7). ERG expression is not restricted to development. In adult mouse, ERG is normally expressed in endothelial tissues, including adrenal, cartilage, heart, spleen, lymphatic endothelial and eosinophil cells (8). However, deregulation of ERG activity, often resulting from chromosomal rearrangements, has been implicated and linked to poor prognosis in a number of different cancers. Chromosomal translocations generating EWS/ERG chimeric proteins comprised of the amino-terminal transactivation domain of Ewing’s sarcoma breakpoint region 1 (EWS) and the carboxy-terminal ETS domain of ERG have been identified in 5-10% of Ewing’s sarcoma, an aggressive bone and soft tissue tumor (9). Chromosomal translocations between ERG and TLS/FUS or ERG and ELF4 have been implicated in acute myeloid leukemia (10, 11). Over-expression of ERG, resulting from gene fusion with the androgen-driven promoter of the TMPRSS2 gene, has been identified as a key driver of metastasis and marker for poor prognosis in prostate cancer (12). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
| 参考文献 | 1 . Adamo, P. and Ladomery, M.R. (2016) Oncogene 35, 403-14. 2 . Birdsey, G.M. et al. (2008) Blood 111, 3498-506. 3 . Vijayaraj, P. et al. (2012) Development 139, 3973-85. 4 . Ng, A.P. et al. (2011) Blood 118, 2454-61. 5 . Birdsey, G.M. et al. (2015) Dev Cell 32, 82-96. 6 . Lathen, C. et al. (2014) Circulation 130, 1179-91. 7 . McLaughlin, F. et al. (2001) Blood 98, 3332-9. 8 . Mohamed, A.A. et al. (2010) J Cancer 1, 197-208. 9 . Chen, S. et al. (2016) Genes Chromosomes Cancer 55, 340-9. 10 . Ichikawa, H. et al. (1994) Cancer Res 54, 2865-8. 11 . Moore, S.D. et al. (2006) Leuk Res 30, 1037-42. 12 . Tomlins, S.A. et al. (2005) Science 310, 644-8. |
Immunohistochemical analysis of paraffin-embedded normal human spleen using ERG (A7L1G) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma (two different cases) using ERG (A7L1G) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded MOLT-4 (left), VCAP (middle), and LNCaP (right) cell pellets using ERG (A7L1G) Rabbit mAb. As expected LNCaP cells are negative for ERG expression. | |
Immunoprecipitation of ERG from MOLT-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ERG (A7L1G) Rabbit mAb. Western blot analysis was performed using ERG (A7L1G) Rabbit mAb. | |
Western blot analysis of extracts from MOLT-4, LNCaP, and MS-1 cell lines using ERG (A7L1G) Rabbit mAb. As expected LNCaP cells are negative for ERG expression. | |
Confocal immunofluorescent analysis of MOLT-4 (left) and A-204 (right) cells using ERG (A7L1G) Rabbit mAb (green). Actin filaments have been labeled with DyLight™ 554 Phalloidin (red). As expected, A-204 cells are negative for ERG expression. | |
Flow cytometric analysis of LNCaP (blue) and MOLT-4 (green) cells using ERG (A7L1G) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
