| 货号 | 2144S |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | The α-Tubulin Antibody detects endogenous levels of total α-tubulin protein, and does not cross-react with recombinant β-tubulin. |
| 使用方法 | WB(1:1000) IHC-P (1:50) F (1:25) IF-IC (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| 存放说明 | -20C |
| 计算分子量 | 52 |
| 参考文献 | 1 . Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47. |
Western blot analysis of extracts from CAD and C6 cells, using α-Tubulin Antibody. 使用α-Tubulin Antibody，免疫印迹（Western Blot）分析CAD和C6细胞中α-Tubulin蛋白水平。 | |
Western blot analysis of recombinant α-tubulin and β-tubulin GST-fusion proteins, and extracts from CAD cells, using α-Tubulin Antibody (left), β-Tubulin Antibody #2146 (middle) and GST Antibody #2622 (right). 使用α-Tubulin Antibody (左图)、β-Tubulin Antibody #2146 (中间图)和GST Antibody #2622 (右图)，免疫印迹（Western Blot）分析CAD细胞中重组的α-Tubulin和β-tubulin GST-fusion蛋白水平。 | |
Flow cytometric analysis of C6 cells, using α-Tubulin Antibody (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (红色)比较，使用α-Tubulin Antibody (蓝色)标记进行流式细胞仪分析C6细胞。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using α-Tubulin Antibody. 使用α-Tubulin Antibody，免疫组化分析人源乳腺癌组织石蜡切片，结果显示定位细胞质。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using α-Tubulin Antibody. 使用α-Tubulin Antibody，免疫组化分析人源结肠癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkins lymphoma, using α-Tubulin Antibody. 使用α-Tubulin Antibody，免疫组化分析人源Non-Hodgkins lymphoma组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using α-Tubulin Antibody in the presence of control peptide (left) or antigen-specific peptide (right). 使用α-Tubulin Antibody，并且提前与control peptide (左图)或antigen-specific peptide (右图)孵育，免疫组化分析人源乳腺癌组织石蜡切片。 | |
Confocal immunofluorescent analysis of NIH/3T3 cells, using α-Tubulin Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用α-Tubulin Antibody (绿色)标记，共聚焦免疫荧光观察α-Tubulin蛋白在NIH/3T3细胞中定位。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。蓝色伪彩=DRAQ5®#4084(DNA荧光染料)。 |
