| 货号 | 2148S |
| 反应种属 | Human/Mouse/Rat/Monkey/Zebrafish/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | The α/β-Tubulin Antibody detects endogenous levels of both α- and β-tubulin total protein, and does cross-react with both recombinant α- and β-tubulin. |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47. |
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS-7 cells, using α/β-Tubulin Antibody. 使用α/β-Tubulin Antibody，免疫印迹（Western Blot）分析HeLa、NIH/3T3、C6和COS-7细胞中α/β-Tubulin蛋白水平。 | |
Western blot analysis of recombinant alpha-tubulin and beta-tubulin GST-fusion proteins, and extracts from NIH/3T3 and C6 cells, using α/β-Tubulin Antibody (left) and GST Antibody #2622 (right). 使用α/β-Tubulin Antibody (左图)和GST Antibody #2622 (右图)，免疫印迹（Western Blot）分析NIH/3T3和C6细胞中重组的alpha-tubulin and beta-tubulin GST-fusion蛋白水平。 | |
Flow cytometric analysis of C6 cells using α/β-Tubulin Antibody (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (红色)比较，使用α/β-Tubulin Antibody (蓝色)标记进行流式细胞仪分析C6细胞。 | |
Confocal microscopic images of NIH/3T3 cells showing cytoskeletal stain with α/β-Tubulin Antibody (A) compared to an isotype control (B). 与an isotype control (B)比较，使用α/β-Tubulin Antibody (A)，共聚焦免疫荧光观察NIH/3T3细胞，结果显示细胞骨架着色。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using α/β-Tubulin Antibody. 使用α/β-Tubulin Antibody，免疫组化分析人源结肠癌组织石蜡切片，结果显示细胞质定位。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using α/β-Tubulin Antibody. 使用α/β-Tubulin Antibody，免疫组化分析人源乳腺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human MALToma using α/β-Tubulin Antibody. 使用α/β-Tubulin Antibody，免疫组化分析人源淋巴瘤组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb #2128 in the presence of control peptide (left) or β-Tubulin Blocking Peptide (right). 使用β-Tubulin (9F3) Rabbit mAb #2128，并且提前与control peptide (左图)或β-Tubulin Blocking Peptide (右图)孵育，免疫组化分析人源乳腺癌组织石蜡切片。 | |
Confocal immunofluorescent analysis of NIH-3T3 cells, using α/β-Tubulin Antibody (green) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (red) showing different stages of the cell cycle. 使用α/β-Tubulin Antibody (绿色)和Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (红色)，共聚焦免疫荧光观察NIH/3T3细胞，结果显示细胞周期的不同阶段。 |
