| 货号 | 2648S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | This antibody detects endogenous levels of total VCP protein. |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Valosin-containing protein (VCP) is a highly conserved and abundant 97 kDa protein that belongs to the AAA (ATPase associated with a variety of cellular activities) family of proteins. VCP assembles as a homo-hexamer, forming a ring with a channel at its center (1,2,3). VCP homo-hexamers associate with a variety of protein cofactors to form many distinct protein complexes, which act as chaperones to unfold proteins and transport them to specific cellular compartments or to the proteosome (4). These protein complexes participate in many cellular functions, including vesicle transport and fusion, fragmentation and reassembly of the golgi stacks during mitosis, nuclear envelope formation and spindle disassembly following mitosis, cell cycle regulation, DNA damage repair, apoptosis, B- and T-cell activation, NF-κB-mediated transcriptional regulation, endoplasmic reticulum (ER)-associated degradation and protein degradation (4). VCP appears to localize mainly to the endoplasmic reticulum; however, tyrosine phosphorylation is associated with relocalization to the centrosome during mitosis (5). In addition, following cellular exposure to ionizing radition, VCP is phosphorylated at Ser784 in an ATM-dependent manner and accumulates in the nucleus at sites of double-stranded DNA breaks (DSBs) (6). Exposure to other types of DNA damaging agents such as UV light, bleomycin or doxorubicin results in phosphorylation of VCP by ATR and DNA-PK in an ATM-independent manner (6).含缬酪肽蛋白(VCP)是一个高度保守和丰度高的97 kDa蛋白质，属于AAA(ATP酶关联的多种细胞活动)蛋白家族。VCP组装成同源六聚体，在其中心通道处形成一个环(1,2,3)。VCP同源六聚体连同多种蛋白辅因子形成许多不同的蛋白复合体，作为分子伴侣展开蛋白质，并将它们传送到特定的细胞区室或蛋白酶体(4)。这些蛋白复合体参与多种细胞功能，包括囊泡运输和融合，有丝分裂高尔基体破碎和重组，伴随有丝分裂的核包膜的形成和纺锤体的拆卸，细胞周期调控，DNA损伤修复，细胞凋亡，B-和T-细胞激活， NF-κB介导的转录调控，内质网（ER）相关的降解和蛋白质降解（4）。VCP似乎主要定位于内质网；然而，酪氨酸磷酸化与其有丝分裂期中心体再定位有关(5)。此外，细胞暴露于电离辐射后，VCP的第784位丝氨酸发生ATM依赖方式的磷酸化，并在细胞核中的DNA双链断裂处累积(6)。暴露于其它类型的DNA损伤剂如紫外线、博来霉素或阿霉素将导致经由ATR 和DNA-PK的ATM依赖的VCP磷酸化(6)。 |
| 存放说明 | -20C |
| 计算分子量 | 89 |
| 参考文献 | 1 . DeLaBarre, B. and Brunger, A.T. (2003) Nat. Struct. Biol. 10, 856-863. 2 . Huyton, T. et al. (2003) J. Struct. Biol. 144, 337-348. 3 . Dreveny, I. et al. (2004) EMBO J. 23, 1030-1039. 4 . Wang, Q. et al. (2004) J. Struct. Biol. 146, 44-57. 5 . Madeo, F. et al. (1998) Mol. Biol. Cell 9, 131-141. 6 . Livingstone, M. et al. (2005) Cancer Res. 65, 7533-7540. |
Western blot analysis of extracts from HeLa, K562, NIH/3T3, C6 and COS cells, using VCP Antibody.Western blot 方法检测HeLa, K562, NIH/3T3, C6 和COS细胞提取物，使用的抗体为VCP Antibody。 | |
Immunofluorescence staining of paraformaldehyde-fixed HeLa cells, using VCP Antibody.免疫荧光染色方法检测甲醛固定的Hela细胞，使用的抗体为VCP Antibody。 | |
Flow cytometric analysis of K562 cells, using VCP Antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞术检测K562细胞，使用的抗体为 VCP Antibody（蓝色），非特异性阴性对照抗体作为对照（红色）。 |
