| 货号 | 2502S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | Vav Antibody detects endogenous levels of total Vav1 protein. The antibody does not cross-react with Vav2, Vav3 or CelVav. |
| 使用方法 | WB(1:1000) F (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
| 参考文献 | 1 . Hornstein, I. et al. (2004) Cell. Signal. 16, 1-11. 2 . Crespo, P. et al. (1997) Nature 385, 169-172. 3 . Han, J. et al. (1997) Mol. Cell. Biol. 17, 1346-1353. |
Flow cytometric analysis of untreated Jurkat cells using Vav1 antibody (blue) compared to a nonspecific negative control antibody (red). 与一个非特异阴性control antibody (红色)比较，使用Vav1 antibody (蓝色)进行流式细胞仪分析未处理的Jurkat细胞。 | |
Western blot analysis of extracts from various cell lines using Vav1 Antibody. |
