| 货号 | 4890S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | UBE1a Antibody detects endogenous levels of total UBE1a protein. This antibody does not cross-react with UBE1b. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:175) F (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | 泛素是一个在泛素——蛋白酶体途径中发挥重要作用的保守多肽。泛素能够通过泛素转移过程被共价修饰于多个细胞蛋白，导致蛋白通过26S蛋白酶体降解。三个泛素化酶参与泛素偶联过程：泛素首先与活化酶E1(UBE1或E1)形成硫酯键复合体而被激活，然后被转移到泛素载体蛋白E2，然后由E2转移到E3并连接到靶蛋白赖氨酸的epsilon位氨基上(1-3)，不同E2和E3的组合决定底物特异性(4)。UBE1有两个亚型：UBE1a是一个117kDa的核蛋白，UBE1b是一个110kDa的核质蛋白(5). |
| 存放说明 | -20C |
| 计算分子量 | 117 |
| 参考文献 | 1 . Ciechanover, A. (1998) EMBO J 17, 7151-60. 2 . Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7. 3 . Hochstrasser, M. (2000) Science 289, 563-4. 4 . DeSalle, L.M. and Pagano, M. (2001) FEBS Lett 490, 179-89. 5 . Stephen, A.G. et al. (1996) J. Biol. Chem. 271, 15608-15614. |
Flow cytometric analysis of K562 cells using UBE1a Antibody (blue) compared to a nonspecific negative control antibody (red). | |
Confocal immunofluorescent analysis of SK-N-MC cells using UBE1a Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). | |
Western blot analysis of extracts from K562 and SK-N-MC cells using UBE1a Antibody. | |
Immunohistochemical analysis of paraffin-embedded mouse brain using UBE1a Antibody in the presence of control peptide (left) or antigen specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using UBE1a Antibody. | |
Immunohistochemical analysis of paraffin-embedded glioblastoma using UBE1a Antibody. |
