| 货号 | 3694S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 目标/特异性 | TRADD Antibody detects endogenous levels of total TRADD protein. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Apoptosis mediated by death factors like FasL and TNF-α involves the formation of a death-inducing signaling complex (DISC) to their respective receptors (1). Upon ligand activation to their receptors, Fas and TNF-R1 associate with death domain (DD) containing adaptor proteins FADD (Fas associated death domain) (2,3) and TRADD (TNF-R1 associated death domain) (4). In addition to its carboxy-terminal DD, FADD contains an amino-terminal death effector domain (DED) that binds to DEDs found on caspase-8 which leads to activation of this initiator caspase (5,6). Caspase-8 subsequently activates downstream effector caspases, like caspase-3, resulting in the cleavage of proteins involved in the execution of apoptosis. Unlike FADD, TRADD does not contain a DED (4). Apoptosis driven by TNF-R1 binding to TRADD involves association of TRADD and FADD which then leads to activation of caspase-8 (7). |
| 存放说明 | -20C |
| 计算分子量 | 34 |
| 参考文献 | 1 . Nagata, S. (1997) Cell 88, 355-65. 2 . Chinnaiyan, A.M. et al. (1995) Cell 81, 505-12. 3 . Boldin, M.P. et al. (1995) J Biol Chem 270, 7795-8. 4 . Hsu, H. et al. (1995) Cell 81, 495-504. 5 . Muzio, M. et al. (1996) Cell 85, 817-27. 6 . Boldin, M.P. et al. (1996) Cell 85, 803-15. 7 . Hsu, H. et al. (1996) Cell 84, 299-308. |
Western blot analysis of extracts from MCF-7 (human), C2C12 (mouse) and KNRK (rat) cell lines, using TRADD Antibody. Western blot 检测MCF-7 （人）、C2C12（小鼠）和KNRK（大鼠）细胞系提取物，使用抗体为TRADD Antibody。 | |
Flow cytometric analysis of HT29 cells, using TRADD antibody (blue) compared to a nonspecific negative control antibody (red). 流式细胞仪检测HT29细胞，使用抗体为TRADD antibody（蓝色）和一个非特异性阴性对照抗体（红色）。 |
