| 货号 | 4505S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | TAK1 Antibody detects endogenous levels of total TAK1 protein. |
| 使用方法 | WB(1:1000) F (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2). |
| 存放说明 | -20C |
| 计算分子量 | 78 to 82 |
| 参考文献 | 1 . Yamaguchi, K. et al. (1995) Science 270, 2008-11. 2 . Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6. 3 . Shibuya, H. et al. (1996) Science 272, 1179-82. 4 . Sakurai, H. et al. (2000) FEBS Lett 474, 141-5. 5 . Takaesu, G. et al. (2000) Mol Cell 5, 649-58. 6 . Onodera, Y. et al. (2015) FEBS Open Bio 5, 492-501. |
Flow cytometric analysis of HeLa cells, using Tak1 Antibody (blue) compared to a nonspecific negative control antibody (red).Flow cytometric方法检测细胞提取物：HeLa 细胞，使用的抗体是Tak1 Antibody（蓝色），红色曲线表示非特异性阴性对照。 | |
Western blot analysis of extracts from untreated or IL-1 treated 293-IL-1R cells, and extracts from HeLa, NIH/3T3, C6, and COS cells, using TAK1 Antibody.western blot方法检测细胞提取物：未处理和 IL-1处理的293-IL-1R细胞以及HeLa, NIH/3T3, C6, COS细胞。使用的抗体是TAK1 Antibody。 |
