| 货号 | 3347S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | SYT1 Antibody detects endogenous levels of total SYT1 protein. |
| 使用方法 | WB(1:1000) F (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Synaptotagmin 1 (SYT1) is an integral membrane protein found in synaptic vesicles thought to play a role in vesicle trafficking and exocytosis (1). Individual SYT1 proteins are composed of an amino-terminal transmembrane region, a central linker region and a pair of carboxy-terminal C2 domains responsible for binding Ca2+ (2). The C2 domains appear to be functionally distinct, with the C2A domain responsible for regulating synaptic vesicle fusion in a calcium-dependent manner during exocytosis while the C2B domain allows for interaction between adjacent SYT1 proteins (3). Because synaptotagmin 1 binds calcium and is found in synaptic vesicles, this integral membrane protein is thought act as a calcium sensor in fast synaptic vesicle exocytosis. Evidence suggests possible roles in vesicle-mediated endocytosis and glucose-induced insulin secretion as well (4,5). SYT1 binds several different SNARE proteins during calcium-mediated vesicle endocytosis and an association between SYT1 and the SNARE protein SNAP-25 is thought to be a key element in vesicle-mediated exocytosis (6). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Fukuda, M. and Mikoshiba, K. (2001) Biochem Biophys Res Commun 281, 1226-33. 2 . Südhof, T.C. (2002) J Biol Chem 277, 7629-32. 3 . Fernández-Chacón, R. et al. (2001) Nature 410, 41-9. 4 . Lynch, K.L. et al. (2007) Mol Biol Cell 18, 4957-68. 5 . Gauthier, B.R. and Wollheim, C.B. (2008) Am J Physiol Endocrinol Metab 295, E1279-86. 6 . Bai, J. et al. (2004) Neuron 41, 929-42. |
Western blot analysis of extracts from various cell lines using Synaptotagmin-1 Antibody. | |
Flow cytometric analysis of C2C12 cells using Synaptotagmin-1 Antibody (blue) compared to a nonspecific negative control antibody (red). |
