| 货号 | 2108S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Src Antibody detects endogenous levels of Src proteins. This antibody may cross-react with other Src family members, but does not cross-react with other non-family proteins. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609. 2 . Hunter, T. (1987) Cell 49, 1-4. |
Western blot analysis of extracts from various cell lines using Src Antibody. | |
采用Src Antibody兔多抗，对多种细胞系的提取物进行Western blot检测。 | |
