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货号： bs-1152R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1152R
- 英文名称
- ATPase Na+/ K+ beta 2
- 中文名称
- 钠钾ATP酶通道蛋白抗体
- 别 名
- adhesion molecule on glia; Na+K+ATPase; AMOG; AT1B2; AT1B2_HUMAN; ATP1B2; ATPase Na+/K+ transporting beta 2 polypeptide; ATPB2; ATPB2S; MGC93648; Na+/K+ -ATPase beta 2 subunit; Na, K ATPase beta 2 polypeptide; RATATPB2S; sodium potassium ATPase subunit beta 2 (non-catalytic); sodium pump subunit beta 2; sodium/potassium dependent ATPase beta 2 subunit; Sodium/potassium dependent ATPase subunit beta 2; sodium/potassium transporting ATPase beta 2 chain; sodium/potassium transporting ATPase subunit beta 2; Sodium/potassium-dependent ATPase subunit beta-2; Sodium/potassium-transporting ATPase subunit beta-2.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 通道蛋白 糖蛋白
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 33kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human ATP1b2:201-290/290 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. [provided by RefSeq, Jul 2008]
Function:
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known.
Subunit:
Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular Location:
Membrane; Single-pass type II membrane protein.
Similarity:
Belongs to the X(+)/potassium ATPases subunit beta family.
SWISS:
P14415
Gene ID:
482
Database links:Entrez Gene: 482Human
Omim: 182331Human
SwissProt: P14415Human
Unigene: 643540Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
通道蛋白（Channel Protein）
钠钾ATP酶是位于细胞膜上的一种糖蛋白,与ATP 的分解和细胞内外钠、钾离子的转运密切相关,哺乳动物各种组织细胞的钠钾ATP 酶的免疫学特性基本相同。
- 产品图片
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-ATPase Na+/ K+ beta 2 (bs-1152R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Sample: NIH/3T3(mouse) Cell Lysate at 40 ug
Primary: Anti-ATPaseNa＋/K＋ beta2 (bs-1152R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AMOG Polyclonal Antibody, Unconjugated(bs-1152R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ATP1b2/Na+K+ATPase Polyclonal Antibody, Unconjugated(bs-1152R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining