| 货号 | 2564S |
| 反应种属 | Human/Mouse/Rat/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | RhoGDI Antibody detects endogenous levels of total RhoGDI protein. |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI). RhoGDI affects Rho activity by inhibiting nucleotide exchange and membrane association, regulating activity and localization (Reviewed in 1, 2). The inhibitory and shuttling functions of RhoGDI have been uncoupled using mutant forms of RhoGDI (3). Phosphorylation of GDIs and/or GTPases can modulate their affinity for each other and, therefore, GTPase mediated signaling. PAK1 phosphorylation of RhoGDI at serines 101 and 174 causes release and activation of Rac1, but not RhoA (4). |
| 存放说明 | -20C |
| 计算分子量 | 26 |
| 参考文献 | 1 . DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol. 15, 356-363. 2 . Dovas, A. and Couchman, J.R. (2005) Biochem. J. 390, 1-9. 3 . Dransart, E. et al. (2005) J Biol Chem 280, 4674-83. 4 . DerMardirossian, C. et al. (2004) Mol Cell 15, 117-27. |
Western blot analysis of HeLa, NIH/3T3 and C6 cells, using RhoGDI Antibody. 使用RhoGDI Antibody，免疫印迹（Western Blot）分析HeLa、NIH/3T3和C6细胞中RhoGDI蛋白水平。 | |
Flow cytometric analysis of HeLa cells, using RhoGDI Antibody (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (红色)比较，使用RhoGDI Antibody (蓝色)进行流式细胞仪分析HeLa细胞。 | |
Confocal immunofluorescent analysis of MCF-7 cells using RhoGDI Antibody (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
