| 货号 | 4938S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | Rap1 Antibody detects endogenous levels of total Rap1A and Rap1B protein. This antibody does not cross-react with Rap2 and other Ras-related proteins. |
| 使用方法 | WB(1:1000) F (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Rap1 and Rap2 belong to the Ras subfamily of small GTPases and are activated by a wide variety of stimuli through integrins, receptor tyrosine kinases (RTKs), G-protein coupled receptors (GPCR), death domain associated receptors (DD-R) and ion channels (1,2). Like other small GTPases, Rap activity is stimulated by guanine nucleotide exchange factors (GEF) and inactivated by GTPase activating proteins (GAP). A wide variety of Rap GEFs have been identified: C3G connects Rap1 with RTKs through adaptor proteins such as Crk, Epacs (or cAMP-GEFs) transmit signals from cAMP, and CD-GEFs (or CalDAG-GEFs) convey signals from either or both Ca2+ and DAG (1). Rap1 primarily regulates multiple integrin-dependent processes such as morphogenesis, cell-cell adhesion, hematopoiesis, leukocyte migration and tumor invasion (1,2). Rap1 may also regulate proliferation, differentiation and survival through downstream effectors including B-Raf, PI3K, RalGEF and phospholipases (PLCs) (1-4). Rap1 and Rap2 are not fuctionally redundant as they perform overlapping but distinct functions (5). Recent research indicates that Rap2 regulates Dsh subcellular localization and is required for Wnt signaling in early development (6). |
| 存放说明 | -20C |
| 计算分子量 | 21 |
| 参考文献 | 1 . Bos, J.L. et al. (2001) Nat Rev Mol Cell Biol 2, 369-77. 2 . Caron, E. (2003) J Cell Sci 116, 435-40. 3 . Song, C. et al. (2002) Oncogene 21, 8105-13. 4 . Rong, R. et al. (2003) J Biol Chem 278, 52497-503. 5 . Taira, K. et al. (2004) J Biol Chem 279, 49488-96. 6 . Choi, S.C. and Han, J.K. (2005) EMBO J 24, 985-96. |
Flow cytometric analysis of untreated A431 cells, using Rap1A/Rap1B antibody (blue) compared to a nonspecific negative control antibody (red). 与一个非特异阴性control antibody (红色)比较，使用Rap1A/Rap1B antibody (蓝色)标记，流式细胞仪分析未处理的A431细胞。 | |
Western blot analysis of extracts from HEK293, NIH/3T3, C6 and COS cells, using Rap1A/Rap1B Antibody. 使用Rap1A/Rap1B Antibody，免疫印迹（Western Blot）分析HEK293、NIH/3T3、C6 和COS细胞中Rap1A/Rap1B蛋白水平。 |
