| 货号 | 2465S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | Rac1/2/3 Antibody detects endogenous levels of total Rac1/2/3 proteins. This antibody does not cross-react with other small GTPases. |
| 使用方法 | WB(1:1000) F (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development (1). GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the proteins intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 21 |
| 参考文献 | 1 . Wennerberg, K. and Der, C.J. (2004) J. Cell Sci. 117, 1301-1312. 2 . Bernards, A. and Settleman, J. (2004) Trends Cell Biol 14, 377-85. 3 . Rossman, K.L. et al. (2005) Nat Rev Mol Cell Biol 6, 167-80. |
Flow cytometric analysis of Jurkat cells, using Rac1/2/3 Antibody (blue) compared to a nonspecific negative control antibody (red). 与一个非特异阴性control antibody (红色)标记，使用Rac1/2/3 Antibody (蓝色)进行流式细胞仪分析Jurkat细胞。 | |
Western blot analysis of extracts from COS, C6, NIH/3T3 and HEK293 cells, using Rac1/2/3 Antibody. 使用Rac1/2/3 Antibody，免疫印迹（Western Blot）分析COS、C6、NIH/3T3和HEK293细胞中Rac1/2/3蛋白水平。 |
