| 货号 | 3237S |
| 反应种属 | Human/Mouse/Rat/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Profilin-1 Antibody detects endogenous levels of total profilin-1 protien. The antibody may cross-react with profilin-2. |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The dynamic polymerization and depolymerization of actin filaments, a process governed by external and internal signaling events, is vital for cell motility (immune cell function, migration, invasion, metastasis, angiogenesis), cell division and adhesion. Among the many regulators of actin dynamics are profilins. Profilins are conserved actin binding proteins that affect the rate of actin polymerization by binding actin monomers and promoting the exchange of ADP for ATP (reviewed in 1). Profilins bind to proteins involved in the regulation of actin dynamics including palladin (2), dynamin-1 (3), VASP (4) and N-WASP (5). In mice, knockout of the ubiquitously expressed profilin-1 indicates that the protein is essential for embryonic development (6). Profilin-2 is primarily expressed in brain and functions in the regulation of neurite outgrowth (7), membrane trafficking and endocytosis (3). The recently cloned profilin-3 is expressed in kidney and testes (8). |
| 存放说明 | -20C |
| 计算分子量 | 15 |
| 参考文献 | 1 . Witke, W. (2004) Trends Cell Biol 14, 461-9. 2 . Boukhelifa, M. et al. (2006) FEBS J 273, 26-33. 3 . Gareus, R. et al. (2006) J Biol Chem 281, 2803-11. 4 . Reinhard, M. et al. (1995) EMBO J 14, 1583-9. 5 . Suetsugu, S. et al. (1998) EMBO J 17, 6516-26. 6 . Witke, W. et al. (2001) Proc Natl Acad Sci U S A 98, 3832-6. 7 . Da Silva, J.S. et al. (2003) J Cell Biol 162, 1267-79. 8 . Hu, E. et al. (2001) Exp Nephrol 9, 265-74. |
Western blot analysis of extracts from various cell lines, using Profilin-1 Antibody. 使用Profilin-1 Antibody，免疫印迹（Western Blot）分析不同细胞中Profilin-1蛋白水平。 | |
Confocal immunofluorescent images of C2C12 cells labeled wtih Profilin-1 Antibody (green, left) compared to an isotype control (right). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 与an isotype control (右图)比较，使用Profilin-1 Antibody (绿色,左图)标记，共聚焦免疫荧光分析C2C12细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5™ (DNA荧光染料)。 | |
Flow cytometric analysis of HeLa cells, using Profilin-1 antibody (blue) compared to a nonspecific negative control antibody (red). 与a nonspecific negative control antibody(红色)比较，使用Profilin-1 antibody (蓝色)标记，流式细胞仪分析HeLa细胞。 |
