| 货号 | 9694S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | PIP4K2B Antibody recognizes endogenous levels of total PIP4K2B protein. |
| 使用方法 | WB(1:1000) F (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphatidylinositol-5-phosphate 4-kinases (PIP4K) synthesize phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2), a key precursor in phosphoinositide signaling that directly modulates the activity of signaling proteins and cellular processes. There are two subfamilies of PIP kinases, type I and II, that generate PtdIns(4,5)P2 from distinct substrate pools. PIP4 type I kinases use PtdIns5P as a substrate, whereas PIP5 type II kinases use PtdIns4P (1,2). In mammalian cells, three isoforms of each PIP4K and PIP5K subfamily, encoded by distinct genes, have been characterized (3-7). All PIP kinases are stimulated by phosphatidic acid, extensively regulated by ARF and Rho GTPases, and inhibited by protein kinase A and PI-stimulated autophosphorylation (8). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Rameh, L.E. et al. (1997) Nature 390, 192-6. 2 . Zhang, X. et al. (1997) J Biol Chem 272, 17756-61. 3 . Ishihara, H. et al. (1996) J Biol Chem 271, 23611-4. 4 . Loijens, J.C. and Anderson, R.A. (1996) J Biol Chem 271, 32937-43. 5 . Ishihara, H. et al. (1998) J Biol Chem 273, 8741-8. 6 . Itoh, T. et al (1998) J. Biol. Chem. 273, 20292-20299 7 . Boronenkov, I.V. et al. (1998) Mol Biol Cell 9, 3547-60. 8 . Oude Weernink, P.A. et al. (2004) Eur J Pharmacol 500, 87-99. |
Flow cytometric analysis of HeLa cells using PIP4K2B Antibody (blue) compared to a nonspecific negative control antibody (red). 流失细胞学分析Hela细胞，使用的抗体为PIP4K2B Antibody (蓝色)，对照为一种非特异性阴性对照抗体 (红色) | |
Western blot analysis of extracts from various cell lines using PIP4K2B Antibody. 对多种细胞系抽提物进行Western blot分析，使用的抗体为PIP4K2B Antibody。 |
