| 货号 | 3693S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Perforin Antibody (Mouse Specific) detects endogenous levels of total Perforin protein. |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 70-75 |
Flow cytometric analysis of untreated CTLL-2 cells, using Perforin Antibody (Mouse Specific) (blue) compared to a nonspecific negative control antibody (red). 流式细胞术检测未处理的 CTLL-2 细胞，使用抗体为Perforin Antibody (Mouse Specific)（蓝色），对照一个非特异性阴性对照抗体（红色）。 | |
Western blot analysis of extracts from CTLL-2 cells, using Perforin Antibody (Mouse Specific). Western blot 检测CTLL-2 细胞提取物，使用抗体为Perforin Antibody (Mouse Specific)。 | |
Immunofluorescent analysis of CTLL-2 cells showing granule staining, using Perforin Antibody (Mouse Specific). 免疫荧光检测CTLL-2 细胞，颗粒染色成像，使用抗体为Perforin Antibody (Mouse Specific)。 |
