| 货号 | 4852S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) |
| 供应商 | CST |
| 背景 | Three distinct PCTAIRE isoforms (PCTAIRE 1, PCTAIRE 2 and PCTAIRE 3) have been identified in humans and belong to the CDK family of serine/threonine protein kinases. These proteins have a core kinase domain flanked by unique amino- and carboxy-terminal domains. CDK proteins are known to regulate the cell cycle. All three PCTAIRE isoforms are abundantly expressed and catalytically active in post-mitotic brain, suggesting that they may function in processes other than cell division (1). PCTAIRE 1 is a cytoplasmic phosphoprotein whose kinase activity peaks in G2 and S phase (2). While one study indicates that noncovalent interactions with a regulatory component (such as a cyclin) are necessary for catalytic activity of PCTAIRE 1, others show that the monomeric protein is fully active (3). The Cdk5/p25 complex phosphorylates PCTAIRE 1 at Ser95, enhancing its kinase activity (4).三个不同的PCTAIRE异构体(PCTAIRE 1, PCTAIRE 2 and PCTAIRE 3)已在人类中被鉴别，它们属于CDK丝氨酸/苏氨酸蛋白激酶家族。这些蛋白拥有一个核心的激酶结构域，侧翼是独特的氨基和羧基末端结构域。众所周知，CDK蛋白能够调节细胞周期。所有的三个异构体丰富表达且在脑有丝分裂后被催化激活，表明它们可能在进程中而非细胞分化中发挥功能(1)。PCTAIRE是一个胞质磷蛋白，其激酶活性在G2和S期达到峰值(2)。尽管有一项研究表明与调节部分(如周期蛋白)的非共价相互作用对于PCTAIRE 1的催化活性必不可少，其它的一些研究显示其单体蛋白是充分活化的(3)。Cdk5/p25复合体能够磷酸化PCTAIRE 1第95位丝氨酸，增强其激酶活性(4)。 |
| 存放说明 | -20C |
| 计算分子量 | 65 |
Western blot analysis of extracts from CAD and Neuro2A cells, using PCTAIRE 1 Antibody.Western blot方法检测CAD和Neuro2A细胞提取物，使用的抗体为PCTAIRE 1 Antibody。 | |
Flow cytometric analysis of untreated Neuro 2A cells, using PCTAIRE 1 antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞术检测未处理的Neuro2A细胞，是由的抗体为PCTAIRE 1 Antibody（蓝色），非特异性阴性对照抗体作为对照（红色）。 |
