| 货号 | 2328S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:100) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | PAR-4 (prostate apoptosis response-4) was identified as a protein that is upregulated in prostate tumor cells undergoing apoptosis (1). Additionally, in parallel studies PAR-4 was found in the yeast two-hybrid system to bind to the Wilms tumor suppressor protein WT1 and may modulate WT1-medated transcriptional activation (2). PAR-4 contains a leucine zipper domain and a death domain and has been implicated as an effector of apoptosis during tumorigenesis as well as in neurodegenerative disorders (3,4). PAR-4 is widely expressed in normal tissues but can be downregulated in some tumor types. The mechanism of PAR-4 mediated apoptosis regulation appears to be complex and dependent on the cellular context. Studies have indicated roles for PAR-4 in activation of the Fas-FADD-caspase-8 pathway as well as inhibition of the NF-κB pro-survival pathway (5-7). Its activity is likely to depend on the cellular context and post-translational modifications. For instance, phosphorylation of PAR-4 by Akt prevents its nuclear translocation thereby promoting cell surivival (8). In contrast, phoshorylation of rat PAR-4 at T155 by PKA appears to positively regulate its apoptotic activity (9). |
| 存放说明 | -20C |
| 计算分子量 | 41 |
Flow cytometric analysis of HeLa cells, using PAR-4 Antibody (blue) compared to a nonspecific negative control antibody (red). 流式细胞术检测HeLa细胞，使用的抗体为PAR-4 Antibody （蓝色），对比一个非特异性阴性对照抗体（红色）。 | |
Western blot analysis of extracts from SW620, ACHN, LNCaP and A20 cell lines, using PAR-4 Antibody. Western blot分析SW620、ACHN、LNCaP和A20 细胞的提取物，使用抗体为PAR-4 Antibody。 | |
