| 货号 | 2614S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | This antibody detects endogenous levels of Calcineurin A (alpha isoform). It may also recognize the beta and gamma isoforms of Calcineurin A. The antibody does not cross-react with protein phosphatase 1 or 2A. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:25) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Calcineurin, also known as protein phosphatase 2B (PP2B), is a calmodulin-dependent, calcium-activated, serine/threonine protein phosphatase composed of a catalytic subunit (calcineurin A) and a tightly bound regulatory subunit (calcineurin B) (1). Calcineurin A is highly homologous to protein phosphatases 1 and 2A. Calcineurin B, like calmodulin, contains four EF-hand, calcium-binding motifs. Calcineurin signaling has been implicated in a broad spectrum of cellular processes including cell-cycle regulation, stress response and apoptosis and is required for proper cardiovascular and skeletal muscle development (2,3). Calcineurin-mediated dephosphorylation of the nuclear factor of activated T cells (NFAT) transcription factor is essential for NFAT activation and nuclear translocation and early gene expression in T lymphocytes (2,3). Calcineurin is the target of the immunosuppressive drugs Cyclosporin A and FK506, both of which block the activation of quiescent T cells after T cell receptor engagement (2,3). Cyclosporin A and FK506 bind to the immunophilins, cyclophilin and FKBP respectively and the immunophilin-drug complex binds to calcineurin and blocks substrate binding. |
| 存放说明 | -20C |
| 计算分子量 | 59 |
Western blot analysis of lysates from MCF7, NIH/3T3, C6 and COS cells, using Pan-Calcineurin A antibody. 对MCF7, NIH/3T3, C6和COS细胞裂解液使用Pan-Calcineurin A抗体进行Western blot分析。 | |
Flow cytometric analysis of NIH/3T3 cells, using Pan-Calcineurin A antibody (blue) compared to a nonspecific negative control antibody (red). 使用Pan-Calcineurin A抗体（蓝）和非特异的阴性对照抗体（红），对NIH/3T3细胞进行流式细胞仪分析。 | |
Confocal immunofluorescent analysis of Hela cells, using Pan-Calcineurin A Antibody exhibiting cytoplasmic localization (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HeLa细胞使用Pan-Calcineurin A抗体（绿色）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor® 555 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 |
