| 货号 | 3150S |
| 反应种属 | Human/Mouse/Rat/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Moesin (Q480) Antibody detects endogenous levels of total moesin protein. The antibody does not cross-react with ezrin, radixin or other related proteins. |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:300) |
| 供应商 | CST |
| 背景 | The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
Confocal immunofluorescent images of HeLa cells labeled with Moesin (Q480) Antibody (green, left) compared to an isotype control (right). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 与使用isotype control (右图)比较，使用Moesin (Q480) Antibody (绿色, 左图)标记，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白（红色）。蓝色伪彩= DRAQ5™（DNA荧光染色）。 | |
Flow cytometric analysis of HeLa cells, using Moesin (Q480) Antibody (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (红色)比较，使用Moesin (Q480) Antibody (蓝色)标记，流式细胞仪分析HeLa细胞。 | |
Western blot analysis of extracts from various cells lines, using Moesin (Q480) Antibody. 使用Moesin (Q480) Antibody，免疫印迹（Western Blot）分析不同细胞中Moesin蛋白水平。 |
