| 货号 | 3042S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 使用方法 | WB(1:1000) IP (1:100) F (1:50) |
| 供应商 | CST |
| 背景 | In response to cytokines, stress and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272 and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2). |
| 存放说明 | -20C |
| 计算分子量 | 47, 49 (Phosphorylated). |
Western blot analysis of extracts from untreated or UV treated HeLa and Cos cells using MAPKAPK-2 antibody.Western blot方法检测细胞提取物：未经处理的和紫外照射的HeLa、COS 细胞，使用的抗体是MAPKAPK-2 antibody。 | |
Flow cytometric analysis of Hela cells using MAPKAPK-2 Antibody (blue) compared to a nonspecific negative control antibody (red).Flow cytometric方法检测细胞提取物：HeLa细胞，使用的抗体是MAPKAPK-2 Antibody（蓝色），红色表示非特异性阴性对照抗体。 |
