| 货号 | 4590S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | IHC-P/IF-F/IF-IC/F |
| 目标/特异性 | Insulin Antibody detects endogenous levels of total insulin protein. |
| 使用方法 | IHC-P(1:100) F (1:100) IF-F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into the skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2). |
| 存放说明 | -20C |
| 计算分子量 | 6 |
Immunohistochemical analysis of paraffin-embedded mouse pancreas, showing staining of β cells, using Insulin Antibody. 免疫组织化学检测小鼠石蜡包埋的的胰腺组织，所用抗体为Insulin Antibody,显示beta细胞的染色 | |
Immunohistochemical analysis of paraffin-embedded human pancreas, showing staining of β cells, using Insulin Antibody. 免疫组织化学检测人石蜡包埋的的胰腺组织，所用抗体为Insulin Antibody,显示beta细胞的染色 | |
Immunohistochemical analysis of paraffin-embedded rat pancreas, showing staining of β-cells using Insulin Antibody. 免疫组织化学检测大鼠石蜡包埋的的胰腺组织，所用抗体为Insulin Antibody，显示beta细胞的染色 | |
Confocal immunofluorescent analysis of normal mouse pancreas using Insulin Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 激光共聚焦免疫荧光检测正常小鼠胰腺，所用抗体为Insulin Antibody（绿），蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5 #4084). | |
Flow cytometric analysis of untreated β-TC cells, using Insulin Antibody (blue) compared to a nonspecific negative control antibody (red). 流式细胞检测未处理的beta-TC,所用抗体为Insulin Antibody（蓝）, 对照为非特异性阴性对照（红） | |
Confocal immunofluorescent analysis of beta-TC-6 and HeLa cells using Insulin Antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 激光共聚焦免疫荧光检测beta-TC和hela，所用抗体为Insulin Antibody（绿），蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5 #4084). |
