| 货号 | 5443S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/F |
| 目标/特异性 | Ikaros Antibody detects endogenous levels of total Ikaros protein. It is expected to recognize all isforms of Ikaros protein produced by differential splicing. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) F (1:50) |
| 供应商 | CST |
| 背景 | Ikaros family proteins, characterized by the presence of N-terminal zinc finger DNA-binding domain and C-terminal dimerization domain, belong to the Kruppel transcription factor superfamily. Ikaros family includes Ikaros, Aiolos, Helios, and possibly EOS and Pegasus (1). They can form homodimers as well as heterdimers with other members of the Ikaros family. Due to differential splicing, multiple isoforms can be generated and some behave in a dominant negative manner upon dimerization (2). Ikaros, also known as IKZF1 (Ikaros family zinc finger protein 1) and LYF-1 (Lymphoid transcription factor 1), is expressed abundantly in blood cells. Genetic studies in mice demonstrate that Ikaros is important for the normal development of B, T, natural killer, and dendritic cells, and that it functions as a tumor suppressor (3,4). Imbalanced expression of different Ikaros isoforms as well as deletions of Ikaros are associated with hematologic malignancies in humans (5-7). |
| 存放说明 | -20C |
| 计算分子量 | 50-70 |
Western analysis of extracts from various cell lines using Ikaros Antibody.Western免疫印迹。用 Ikaros Antibody研究各种细胞系的细胞提取液。 | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using Ikaros Antibody.免疫组织化学染色分析石蜡包埋人淋巴癌组织。所用抗体为Ikaros Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ikaros Antibody. Note staining of leukocytes.免疫组织化学染色分析石蜡包埋人乳腺癌组织。所用抗体为Ikaros Antibody。注意白细胞的染色。 | |
Flow cytometric analysis of Jurkat cells using Ikaros Antibody (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (red).流式细胞仪分析Jurkat细胞，所用抗体为Ikaros Antibody (蓝色) 与浓度相匹配的Rabbit (DA1E) mAb IgG XP® Isotype Control(红色)对比。 | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using Ikaros Antibody.免疫组织化学染色分析石蜡包埋小鼠脾组织。所用抗体为Ikaros Antibody。 |
