| 货号 | 4869S |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | HSP60 (D85) Antibody detects endogenous levels of total HSP60 protein. This antibody does not cross-react with other HSPs. |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | In both prokaryotic and eukarytic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). It is now clear that a significant amount of HSP60 is also present in the cytosol of many cells and that it is induced by stress, inflammatory and immune responses, autoantibodies correlated with Alzheimers, coronary artery diseases, MS, and diabetes (5-8). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Flow cytometric analysis of HeLa cells using HSP60 (D85) Antibody (blue) compared to a nonspecific negative control antibody (red). 使用HSP60 (D85) Antibody（蓝）和非特异的阴性对照抗体（红），对HeLa细胞进行流式细胞术分析。 | |
Confocal immunofluorescent analysis of HeLa cells using HSP60 (D85) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HeLa细胞使用HSP60 (D85) Antibody（绿色）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor®555 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Western blot analysis of extracts from various cell types using HSP60 (D85) Antibody. 对多个细胞系使用HSP60 (D85) Antibody进行Western blot分析。 |
