| 货号 | 4868S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | HSP40 Antibody detects endogenous levels of total HSP40 protein. This antibody does not cross-react with other HSPs. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | HSP40 and HSP40-like proteins represent a large family of chaperone proteins that are homologous to E. coli DnaJ protein (1). These proteins are classified into three subtypes based on their structures. The common feature of the family is a conserved J domain, which is usually located at the amino terminus of proteins and responsible for their association with HSP70 (1,2). Human HSP40, also known as Hdj1, belongs to subtype II that contain a unique Gly/Phe-rich region (2). HSP40 family proteins bind unfolded proteins, prevent their aggregation, and then deliver them to HSP70 (2,3). Another major function of HSP40 is to stimulate ATPase activity of HSP70, which causes conformational change of the unfolded proteins (4,5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 or components of the protein degradation machinery such as CHIP and BAG-1, which either leads to protein folding or degradation, respectively (6). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
Flow cytometric analysis of HeLa cells, using HSP40 Antibody (blue) compared to a nonspecific negative control antibody (red). 使用HSP40 Antibody（蓝）和非特异的阴性对照抗体（红），对HeLa细胞进行流式细胞术分析。 | |
Western blot analysis of extracts from HeLa, C2C12 and C6 cells, using HSP40 Antibody. 对HeLa、C2C12和C6细胞使用HSP40 Antibody进行Western blot分析。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or heat-treated (right), using HSP40 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 对HeLa细胞，未处理（左）或加热处理（右）使用HSP40 Antibody（绿色）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor®555 phalloidin（红色）标记。 |
