| 货号 | 2442S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | HSP27 Antibody (Rodent Preferred) detects endogenous levels of total HSP27 protein. The antibody does not cross-react with other heat shock proteins. |
| 使用方法 | WB(1:1000) F (1:25) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
Flow cytometric analysis of C2C12 cells using HSP27 Antibody (Rodent Preferred) (blue) compared to a nonspecific negative control antibody (red). 使用HSP27抗体（啮齿类偏好）（蓝）和非特异的阴性对照抗体（红），对C2C12细胞进行流式细胞仪分析。 | |
Confocal immunofluorescent analysis of C2C12 cells using HSP27 Antibody (Rodent Preferred) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对C2C12细胞使用HSP27抗体（啮齿类偏好）（绿色）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor®555 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Western blot analysis of extracts from C2C12, PC12, HeLa and COS cells using HSP27 Antibody (Rodent Preferred). 对C2C12、PC12、HeLa和COS细胞裂解液使用HSP27抗体（啮齿类偏好）进行Western blot分析。 |
