| 货号 | 4944S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The ubiquitin-conjugating (UBC) enzymes HR6A and HR6B are the mammalian orthologues of the Saccharomyces cerevisiae Rad6 gene products (1). In S. cerevisiae, Rad6 facilitates cell cycle progression and ubiquitinates histone H2B (2,3). In vivo phosphorylation of HR6A Ser120 by cyclin-dependent kinases is thought to be important for the coordination and timing of ubiquitination events involved in cell cycle progression (4). In response to DNA damage, HR6A is known to interact physically with p53 and p14ARF, but knockout mice lacking HR6A or HR6B exhibit normal DNA damage responses (5,6). HR6B knockout males exhibit defective spermatogenesis, while HR6A knockout females fail to produce viable offspring (6).泛素结合(UBC)酶HR6A 和HR6B是酿酒酵母Rad6基因在哺乳动物中的同源基因产物(1)。在啤酒酵母中，Rad6促进细胞周期进程和泛素化组蛋白H2B (2,3)。周期蛋白依赖性激酶介导的HR6A Ser120在体磷酸化被认为在参与细胞周期进程的协调和时序性泛素化过程中是很重要的(4)。DNA损伤应答中，HR6A能够与p53 和p14ARF相互作用，但是敲除后的小鼠缺少HR6A 或者 HR6B显示正常的DNA损伤反应(5,6)。HR6B敲除的雄鼠呈现精子发生缺陷，HR6A敲除的雌鼠不能生产有生育能力的后代(6)。 |
| 存放说明 | -20C |
| 计算分子量 | 17 |
Western blot analysis of extracts from HeLa, ME-180, 293 and K562 cells, using HR6A/HR6B Antibody.Western blot 方法检测HeLa, ME-180, 293 和K562 细胞提取物，使用的抗体为HR6A/HR6B Antibody。 | |
Immunofluorescent analysis of ME-180 cells, using HR6A/HR6B Antibody.免疫荧光方法检测ME-180细胞，使用的抗体为HR6A/HR6B Antibody。 | |
Flow cytometric analysis of untreated Jurkat cells, using HR6A/HR6B Antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞术检测未处理的Jurkat细胞，使用的抗体为HR6A/HR6B Antibody（蓝色），非特异性阴性抗体作为对照（红色）。 |
