| 货号 | 2619S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | HP1γ Antibody detects endogenous levels of total HP1gamma protein. The antibody does not cross-react HP1alpha or HP1beta proteins. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure (1). All three HP1 family members (α, β and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome (2,3). HP1 proteins are approximately 25 kDa in size and each contains a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated on Lys9, a histone "mark" closely associated with centromeric heterochromatin (4,5). The variable hinge region binds both RNA and DNA in a sequence-independent manner (6). The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases and the p150 subunit of chromatin-assembly factor-1 (CAF1) (7-9). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells (10,11). HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination and sumoylation, suggesting multiple means of regulation (12-14). |
| 存放说明 | -20C |
| 计算分子量 | 22 |
Flow cytometric analysis of untreated HeLa cells, using HP1 gamma antibody (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (红色)比较，使用HP1 gamma antibody (蓝色)标记，流式细胞仪分析未处理的HeLa细胞。 | |
Western blot analysis of lysates from HeLa, NIH/3T3, C6 and COS cells, using HP1gamma antibody. 使用HP1gamma antibody，免疫印迹(Western blot)分析HeLa、NIH/3T3、C6和COS细胞中HP1gamma的蛋白水平。 | |
Confocal immunofluorescent image of HeLa cells labeled with HP1gamma Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用HP1gamma Antibody (绿色)标记，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin (红色)标记微丝蛋白。蓝色= DRAQ5™ (DNA荧光染料)。 |
