| 货号 | 4675S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | hnRNP K (R332) Antibody detects endogenous level of total hnRNP K protein. |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Heterogeneous nuclear ribonucleoprotein K (hnRNP K) belongs to a family of RNA binding multiprotein complexes (hnRNP proteins) that facilitate pre-mRNA processing and transport of mRNA from the nucleus to cytoplasm (1-3). hnRNP K contains three unique structural motifs termed KH domains that bind poly(C) DNA and RNA sequences (4,5). Intricate architecture enables hnRNP K to facilitate mRNA biosynthesis (6), transcriptional regulation (7) and signal transduction. Cytoplasmic hnRNP K expression is increased in oral squamous cell carcinoma and pancreatic cancer and may be a potential prognostic factor (8,9). hnRNP K coordinates with p53 to regulate its target gene transcription in response to DNA damage. Proteasome degradation of hnRNP K is mediated by E3 ligase MDM2 (10). The interaction between hnRNP K and c-Src leads to hnRNP K phosphorylation, which allows for hnRNP K activation of silenced mRNA translation (11). |
| 存放说明 | -20C |
| 计算分子量 | 58-62 |
Flow cytometric analysis of HeLa cells using hnRNP K (R332) Antibody (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 与Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（红色）比较，使用hnRNP K (R332) Antibody (蓝色)流式细胞术分析HeLa细胞。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using hnRNP K (R332) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用hnRNP K (R332) Antibody（绿色）标记，共聚焦免疫荧光观察hnRNP A1蛋白在HeLa细胞定位，分为紫外未照射组和紫外照射组。DY-554 phalloidin标记微丝蛋白（红色）。 | |
