| 货号 | 3145S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | Ezrin Antibody detects endogenous levels of total ezrin protein. This antibody does not cross-react with ezrin homologues such as radixin and moesin. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6). |
| 存放说明 | -20C |
| 计算分子量 | 81 |
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using Ezrin Antibody.使用Ezrin Antibody，免疫印迹（Western Blot）分析HeLa、NIH/3T3、C6和COS细胞中Ezrin蛋白水平。 | |
Flow cytometric analysis of untreated HeLa cells, using Ezrin Antibody (blue) compared to a nonspecific negative control antibody (red).与非特异阴性control antibody (红色)比较，使用Ezrin Antibody（蓝色）标记，流式细胞仪分析未处理的HeLa细胞。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Ezrin Antibody使用Ezrin Antibody，免疫组化分析人源肺癌组织石蜡切片。. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Ezrin Antibody in the presence of control peptide (left) or antigen-specific peptide (right).使用Ezrin Antibody ，其分别孵育control peptide (左图)或antigen-specific peptide (右图)，免疫组化分析人源乳腺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using Ezrin Antibody.使用Ezrin Antibody，免疫组化分析4T1 syngeneic小鼠肿瘤组织石蜡切片。 | |
Confocal immunofluorescent analysis of HeLa cells labeled with Ezrin Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).使用Ezrin Antibody (绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白（红色）。蓝色= DRAQ5™（DNA荧光染料）。 |
