| 货号 | 2498S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | eIF4G Antibody detects endogenous levels of total eIF4G protein. |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5 m7GTP mRNA cap and unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 220 |
Flow cytometric analysis of HeLa cells, using eIF4G Antibody (blue) compared to a nonspecific negative control antibody (red). 与非特异的阴性control antibody (红色)比较，使用eIF4G Antibody (蓝色)标记，流式细胞术分析HeLa细胞。 | |
Confocal immunofluorescent analysis of HeLa cells, using eIF4G Antibody (green, left) compared to an isotype control (right). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 与an isotype control（右图）比较，使用eIF4G Antibody (绿色，左图)标记，共聚焦免疫荧光观察eIF4G蛋白在HeLa细胞定位。Alexa Fluor® 555 phalloidin (红色)标记微丝蛋白。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料)。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using eIF4G Antibody in the presence of control peptide (left) or eIF4G blocking peptide #1003 (right). 使用eIF4G Antibody，免疫组化分析人类乳腺癌组织石蜡切片，其分别孵育对照多肽(左图)和eIF4G blocking peptide #1003 (右图)。 | |
Western blot analysis of extracts from various cell lines, using eIF4G Antibody. 使用eIF4G Antibody，免疫印迹（Western Blotting）分析不同细胞系提取物。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using eIF4G Antibody. 使用eIF4G Antibody，免疫印迹分析人类结肠癌石蜡切片，结果显示eIF4G蛋白定位在细胞质。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic localization, using eIF4G Antibody. 使用eIF4G Antibody，免疫组化分析人类肺癌组织石蜡切片，结果显示eIF4G蛋白定位在细胞质。 |
