| 货号 | 4604S |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F/F |
| 目标/特异性 | Doublecortin Antibody detects endogenous levels of total doublecortin protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) IF-F (1:400) |
| 供应商 | CST |
| 背景 | Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Flow cytometric analysis of SK-N-MC cells, using Doublecortin antibody (blue) compared to a nonspecific negative control antibody (red). 流式细胞仪分析SK-N-MC细胞，使用Doublecortin antibody 兔多抗（蓝）和非特异的阴性对照抗体（红）进行标记。 | |
Confocal immunofluorescent analysis of Doublecortin Antibody (red) and GFAP (GA5) Mouse mAb #3670 (green) staining the subventricular zone of the striatum in reperfused ischemic rat brain. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from mouse and rat brain, using Doublecortin Antibody. |
