| 货号 | 9407S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | DDX6/RCK Antibody detects endogenous levels of total DDX6/RCK protein. |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | DDX6, also known as RCK and p54, was identified as a proto-oncogene product and is a member of the ATP-dependent DEAD box helicase family (1,2). This protein interacts with translation initiation factor eIF4E in the cytoplasmic P-bodies (3) and represses mRNA translation (4). DDX6 is a component of the miRNA induced silencing complex (miRISC) and interacts with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) proteins in vitro and in vivo (5), functioning in miRNA-mediated translational repression (5). Depletion of DDX6 leads to the disruption of cytoplasmic P-bodies indicating that it is required for P-body formation (5). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
Western blot analysis of extracts from various cell types using DDX6/RCK Antibody. 使用DDX6/RCK Antibody，免疫印迹（Western Blotting）分析不同细胞系裂解物。 | |
Confocal immunofluorescent analysis of UV-treated C2C12 cells using DDX6/RCK Antibody (green) and α-Tubulin (DM1A) Mouse mAb #3873 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光观察DDX6/RCK蛋白在紫外处理的C2C12细胞定位， 使用DDX6/RCK Antibody（绿色），α-Tubulin (DM1A) Mouse mAb #3873（红色）。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料)。 | |
Flow cytometric analysis of HeLa cells using DDX6/RCK Antibody (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性对照抗体（red）比较，使用DDX6/RCK Antibody（blue）进行流式细胞术分析HeLa细胞。 |
