| 货号 | 4612S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 目标/特异性 | CTMP Antibody detects endogenous levels of total CTMP protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:25) |
| 供应商 | CST |
| 背景 | Carboxyl-terminal modulator protein (CTMP) is a negative regulator of Akt and was identified as an Akt binding protein. Akt, also referred to as PKB or Rac, plays a critical role in controlling the balance between survival and apoptosis (1-3). This protein kinase, activated by insulin and various growth and survival factors, functions in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4), and by phosphorylation within the carboxy-terminus at Ser473. CTMP binds to the regulatory domain of Akt at the carboxy terminus of the protein and inhibits phosphorylation on Thr308 and Ser473 (5). |
| 存放说明 | -20C |
| 计算分子量 | 22, 26 |
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using the CTMP Antibody. Western blot分析HeLa, NIH/3T3 和 C6细胞的提取物，所用抗体为CTMP Antibody. | |
Flow cytometric analysis of HeLa cells, using CTMP antibody (blue) compared to a nonspecific negative control antibody (red). 流式细胞术分析HeLa细胞，所用抗体为CTMP antibody (蓝色) 与非特异的阴性对照抗体（红色）作比较。 |
