| 货号 | 4841S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | Cox1 Antibody Antibody detects endogenous levels of total Cox1 protein. |
| 使用方法 | WB(1:1000) F (1:100) |
| 供应商 | CST |
| 背景 | Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression (4). LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock (5), making Cox2 a useful target for therapeutic anti-inflammatory drugs (3). Cox1 and Cox2 also play a role in the neuropathology of Alzheimers disease by potentiating γ-secretase activity and β-amyloid generation (6). |
| 存放说明 | -20C |
| 计算分子量 | 65, 70 |
Western blot analysis of extracts from C2C12 and Neuro2A cells using Cox1 Antibody.Western免疫印迹。用Cox1 Antibody研究C2C12 和 Neuro2A 细胞的细胞提取液。 | |
Flow cytometric analysis of NIH/3T3 cells using Cox1 Antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞仪研究 NIH/3T3 细胞，所用抗体为Cox1 Antibody (蓝色) 与非特异性的阴性对照抗体(红色)。 |
