| 货号 | 3238S |
| 反应种属 | Human/Mouse/Rat/Hamster/Zebrafish/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | Caveolin-1 Antibody detects endogenous levels of caveolin-1 protein. This antibody does not cross-react with caveolin-2 or -3. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:250) F (1:50) IF-IC (1:400) IHC-F (1:1000) |
| 供应商 | CST |
| 背景 | The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2 and -3) have been identified with different tissue distributions. Caveolins form hetero- and homooligomers, which interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins also interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6). |
| 存放说明 | -20C |
| 计算分子量 | 21, 24 |
Western blot analysis of extracts from various cell lines using Caveolin-1 Antibody. 对多个细胞系使用Caveolin-1 Antibody进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Caveolin-1 Antibody. 对石蜡包埋的人肺癌使用Caveolin-1 Antibody进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung using Caveolin-1 Antibody. 石蜡包埋的人肺癌使用Caveolin-1 Antibody进行免疫组化分析。 | |
Flow cytometric analysis of HeLa cells using Caveolin-1 antibody (blue) compared to a nonspecific negative control antibody (red). 使用Caveolin-1 antibody（蓝）和非特异的阴性对照抗体（红），对HeLa细胞进行流式细胞术分析。 | |
Immunohistochemical analysis of paraffin-embedded Non-Hodgkins lymphoma using Caveolin-1 Antibody. 对石蜡包埋的非霍奇金性淋巴瘤使用Caveolin-1 Antibody进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon using Caveolin-1 Antibody. 对石蜡包埋的人结肠使用Caveolin-1 Antibody进行免疫组化分析。 | |
Immunohistochemical analysis of frozen H1650 xenograft showing cytoplasmic and membrane localization, using Caveolin-1 Antibody. 对冰冻的H1650异种移植物使用Caveolin-1 Antibody进行免疫组化分析，可定位于细胞质和细胞膜。 | |
Confocal immunofluorescent analysis of C2C12 cells using Caveolin-1 Antibody (green). Actin filaments have been labeled with Alexa Fluor(R) 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对C2C12细胞使用Caveolin-1 Antibody（绿色）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor(R) 555 phalloidin（红色）标记。蓝色伪彩=DRAQ5®(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using Caveolin-1 Antibody. 对石蜡包埋的4T1同源基因小鼠肿瘤使用Caveolin-1 Antibody进行免疫组化分析。 |
