| 货号 | 2539S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F/F |
| 目标/特异性 | Calpain 2 Large Subunit (M-type) Antibody detects endogenous levels of total calpain 2 (large subunit) protein. The antibody detects full-length calpain 2 as well as calpain 2 autoproteolytically cleaved at serine 20. The antibody does not detect recombinant calpain 1. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:25) IF-F (1:25) |
| 供应商 | CST |
| 背景 | Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated post-transcriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28 (2). Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation (1). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from C2C12 and PC3 cells, using Calpain 2 Large Subunit (M-type) Antibody.使用Calpain 2 Large Subunit (M-type) 抗体对C2C12和PC3细胞提取物进行western blot分析。 | |
Flow cytometric analysis of C2C12 cells, using Calpain 2 Large Subunit (M-type) Antibody (blue) compared to a nonspecific negative control antibody (red).使用Calpain 2 Large Subunit (M-type) 抗体（蓝色）对比非特异性阴性对照抗体（红色）对C2C12细胞进行流式细胞分析。 | |
Confocal immunofluorescent analysis of mouse retina using Calpain 2 Large Subunit (M-type) Antibody (red) and Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Calpain 2 Large Subunit (M-type) 抗体（红色）和Phospho-Tyrosine 小鼠单抗(P-Tyr-100) #9411（绿色）对小鼠视网膜进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5® #4084 （DNA荧光染料）。 |
