| 货号 | 3354S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 目标/特异性 | CACYBP Antibody detects endogenous levels of total CACYBP protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) F (1:100) |
| 供应商 | CST |
| 背景 | Calcyclin-binding protein or Siah-1-interacting protein (CACYBP/SIP) is a component of the ubiquitin E3 ligase complex that also contains Siah1, Skp1, and Ebi (1). CACYBP regulates β-catenin turnover and plays an important role in thymocyte development (2). CACYBP also binds to tubulin and may be involved in cytoskeletal regulation (3,4). It is highly expressed in neurons, and its cellular localization may be regulated by Ca2+ (5,6). Retinoic acid treatment of the neuroblastoma cell line SH-SY5Y induces translocation of CACYBP to the nucleus and seems to be correlated with phosphorylation of CACYBP on serine residues (7). Recent studies also suggest that CACYBP may possess phosphatase activity (8), and that it can bind and dephosphorylate Erk1/2 (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 26 |
Flow cytometric analysis of HeLa cells using CACYBP Antibody (blue) compared to a nonspecific negative control antibody (red).使用CACYBP抗体（蓝色）对HeLa细胞进行流式细胞分析，对照使用非特异性阴性对照抗体（红色）。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CACYBP Antibody.使用CACYBP抗体对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Western blot analysis of extracts from various cell lines using CACYBP Antibody.使用CACYBP抗体对多种细胞提取物进行western blot分析。 |
