| 货号 | 2119S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | This antibody detects endogenous levels of total BRF1 and BRF2 proteins. |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:125) |
| 供应商 | CST |
| 背景 | Butyrate response factor 1 (BRF1; also known as EGF response factor 1 [ERF1], TIS11B, ZFP36L1) and butyrate response factor 2 (BRF2; also known as EGF response factor 2 [ERF2], TIS11D, ZFP36L2) both belong to the TIS11 family of CCCH zinc-finger proteins (1). This family of proteins, which also includes tristetraprolin (TTP), bind to AU-rich elements (ARE) found in the 3-untranslated regions of mRNAs and promote de-adenylation and rapid degradation by the exosome (2,3). These proteins play a critical role in cell growth control by regulating the mRNA turnover of multiple cytokines, growth factors and cell cycle regulators, including GM-CSF, TNFα, IL-2, IL-3 and IL-6 (4,5). Deregulated ARE-mRNA stability can contribute to both inflammation and oncogenic transformation (6-8). Insulin-induced stabilization of ARE-containing transcripts is mediated by Akt/PKB phosphorylation of BRF1 at Ser92, which results in binding by 14-3-3 protein and inactivation of BRF1 (9). |
| 存放说明 | -20C |
| 计算分子量 | 40 to 50, 62 |
Immunofluorescent analysis of DLD-1 cells, using BRF1/2 Antibody. 使用BRF1/2 Antibody，免疫荧光分析DLD-1细胞。 | |
Western blot analysis of cell lysates from HT1080, HT1080 BRF1-/-, HCT15, C6, COS and NIH/3T3 cells, using BRF1/2 Antibody. 使用BRF1/2 Antibody，免疫印迹（Western blot）分析HT1080, HT1080 BRF1-/-, HCT15, C6, COS和NIH/3T3细胞裂解物。 | |
Flow cytometric analysis of A549 cells, using BRF1/2 Antibody (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性对照抗体（red）比较，使用BRF1/2 Antibody（blue）进行流式细胞术分析A549细胞。 |
