| 货号 | 3094S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 使用方法 | WB(1:1000) IP (1:100) F (1:50) |
| 供应商 | CST |
| 背景 | Aurora A (AIK) is a cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5) and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation and altered survivin localization (6).极光激酶A(AIK)是一种调节细胞周期的丝氨酸/苏氨酸蛋白激酶，在多种肿瘤细胞系中过表达(1-3)。在蛋白激酶激活回路中，极光激酶A苏氨酸(288位)的磷酸化可显著提高其活性并靶向有丝分裂过程中蛋白质的蛋白酶体降解(4)。与极光激酶A密切相关的极光激酶B(AIM1)在多重有丝分裂事件中发挥作用，siRNA沉默其表达之后可以导致组蛋白H3磷酸化减少、异常的染色体排列/分离以及生存蛋白的定位改变(6)。 |
| 存放说明 | -20C |
| 计算分子量 | 40 |
Western blot analysis of extracts from Hela, NIH/3T3 and C6 cells, untreated or nocodazole-treated (50 ng/ml, 24 hrs), using Aurora B/AIM1 Antibody.Western blot 方法检测细胞提取物，未处理或者诺考达唑处理(50 ng/ml, 24小时)的Hela，NIH/3T3和C6 细胞，使用的抗体为Aurora B/AIM1 Antibody。 | |
Flow cytometric analysis of Hela cells, using Aurora B/AIM1 Antibody (blue) compared to a nonspecifc negative control antibody (red).流式细胞术分析Hela细胞，蓝色为Aurora B/AIM1 Antibody检测结果，红色为非特异性的阴性对照。 |
