| 货号 | 8057S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | ASCT2 (D7C12) Rabbit mAb recognizes endogenous levels of total ASCT2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Sodium-dependent neutral amino acid transporter type 2 (ASCT2 or SLC1A5) is a neutral amino acid transporter that regulates the uptake of essential amino acids in conjunction with the SLC7A5 bilateral transporter (1,2). ASCT2 appears to be the major glutamine transporter in hepatoma cells and is thought to provide essential amino acids needed for tumor growth (3). Additional evidence suggests that ASCT2 plays a role in activating mTORC1 signaling and is required to suppress autophagy (4,5). Cell surface ASCT2 serves as a receptor for several mammalian interference retroviruses associated with cases of infectious immunodeficiency; variation in a small region of an extracellular loop (ECL2) may be responsible for species-specific differences in receptor function (6). |
| 存放说明 | -20C |
| 计算分子量 | 49, 75 |
Confocal immunofluorescent analysis of HT-29 cells using ASCT2 (D7C12) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测HT-29细胞提取物，所用抗体ASCT2 (D7C12) Rabbit mAb兔单抗(绿色)，蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084) | |
Western blot analysis of extracts from HT-29 cells, untreated or treated with peptide N-glycosidase F (PNGase F), and untreated SW620 cells using ASCT2 (D7C12) Rabbit mAb. Wetern blot检测HT-29细胞提取物，未处理或用N-糖苷酶F处理，以及未处理的SW620细胞。所用抗体为ASCT2 (D7C12) Rabbit mAb兔单抗。 | |
Flow cytometric analysis of HT-29 cells using ASCT2 (D7C12) Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 流式细胞仪检测HT-29细胞，所用抗体为ASCT2 (D7C12) Rabbit mAb兔单抗 (绿色)，对照组所用抗体为浓度匹配的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(红色)。 |
