| 货号 | 8638S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | BATF (D7C5) Rabbit mAb detects endogenous levels of total BATF protein. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:400) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Basic leucine zipper transcriptional factor ATF-like (BATF) is a basic leucine zipper (bZIP) transcription factor and is part of the AP-1/ATF family that forms inhibitory dimers with members of the Jun family (1-3). Expression of BATF is largely restricted with highest levels found in mature T cells, and it is induced in B cells following immune responses including viral infection (1,2). BATF expression is also induced by IL-6 via a Stat3-dependent mechanism (4). BATF plays an important role in the differentiation of immune cell lineages (5-7). Studies of BATF-deficient mice have demonstrated a critical role for BATF in the formation of IL-17-expressing Th17 cells, in part, by regulating the expression of IL-17 (5,6). BATF knockouts are resistant to experimental autoimmune encephalomyelitis (EEA), consistent with the role of Th17 cells in this model for autoimmunity (5). Additional studies have found that BATF is important in generating antibody class switching. BATF is required for the generation of follicular helper T cells (Tfh), by regulating BCL6 and c-Maf (6,7). In B cells, BATF controls the expression of activation-induced cytidine deaminase (AID) and regulates class-switched antibody responses (7). Taken together, these studies suggest that BATF is a key regulator of distinct populations of immune cells. |
| 存放说明 | -20C |
| 计算分子量 | 15 |
Confocal immunofluorescent analysis of KARPAS-299 (left) and HeLa (right) cells using BATF (D7C5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).共聚焦免疫荧光分析KARPAS-299 细胞 (左图) 和HeLa 细胞(右图)，所用抗体为BATF (D7C5) Rabbit mAb (绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。 | |
Western blot analysis of extracts from various cell lines using BATF (D7C5) Rabbit mAb.Western免疫印迹。用BATF (D7C5) Rabbit mAb研究各种细胞的细胞提取液。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct encoding full-length human BATF (hBATF; +), using BATF (D7C5) Rabbit mAb.Western免疫印迹。用BATF (D7C5) Rabbit mAb研究对照转染(-)和转染了表达人全长 BATF 质粒(hBATF; +)的293T细胞的细胞提取液。 | |
Flow cytometric analysis of HeLa (blue) and KARPAS-299 (green) cells using BATF (D7C5) Rabbit mAb.流式细胞仪分析HeLa细胞 (蓝色)和 KARPAS-299 (绿色)，所用抗体为BATF (D7C5) Rabbit mAb。 |
