CISH (D4D9) Rabbit mAb【科瑞奥博】

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CISH (D4D9) Rabbit mAb

货号： 8731S 基本售价： 3360.0 元规格： 100ul

产品信息

概述
货号	8731S
反应种属	Human/Mouse
来源宿主	Rabbit
应用	W/IP/IF-IC/F
目标/特异性	CISH (D4D9) Rabbit mAb recognizes endogenous levels of total CISH protein.
使用方法	WB(1:1000) IP (1:100) F (1:100) IF-IC (1:100)

性能
供应商	CST
背景	The suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway (1-3). The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses.
存放说明	-20C
计算分子量	32, 37

参考图片

Confocal immunofluorescent analysis of NK-92 cells, IL-2 starved for 5 hrs (left) or IL-2 starved for 5 hrs followed by addition of hIL-2 #8907 overnight (right), using CISH (D4D9) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析NK-92 细胞。经过IL-2饥饿5小时 (左图) 或IL-2饥饿5小时然后加入hIL-2 #8907过夜(右图)，所用抗体为CISH (D4D9) Rabbit mAb (绿色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。

Flow cytometric analysis of NK-92 cells, IL-2 starved for 5 hrs (blue) or IL-2 starved for 5 hrs followed by additon of hIL-2 #8907 overnight (green), using CISH (D4D9) Rabbit mAb (green).流式细胞仪分析NK-92细胞，NK-92细胞先经IL-2饥饿5小时 (蓝色) 或经IL-2 饥饿5小时，然后加入hIL-2 #8907 过夜(绿色)，所用抗体为CISH (D4D9) Rabbit mAb (绿色)。

Western blot analysis of extracts from NK-92 cells, untreated (-) or treated (+) with Human Interleukin-2 (hIL-2) #8907 (10 ng/ml, overnight), or BaF3 cells, untreated (-) or treated (+) with Mouse Interleukin-3 (mIL-3) #8923 (10 ng/ml, 6 hr), using CISH (D4D9) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).Western免疫印迹。用CISH (D4D9) Rabbit mAb(上图) 和β-Actin (D6A8) Rabbit mAb #8457 (下图) 研究未经处理(-)的和经hIL-2 #8907 (10 ng/ml)处理过夜(+)的NK-92细胞系的细胞提取液，未经处理(-)的和经mIL-3 #8923 (10 ng/ml, 6 hr)处理(+)的BaF3细胞系的细胞提取液。

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human CISH (hCISH, +), using CISH (D4D9) Rabbit mAb.Western免疫印迹。用CISH (D4D9) Rabbit mAb研究对照转染(-)和转染了表达人全长CISH质粒(hCISH, +)的293T细胞的细胞提取液。